On the Group policy management screen, you need to right-click the Organizational Unit desired and select the option to link an existent GPO.
In our example, we are going to link the group policy named MY-GPO to the root of the domain.
After applying the GPO you need to wait for 10 or 20 minutes.
During this time the GPO will be replicated to other domain controllers.
Reboot a remote computer and verify if the configuration is ignored by the excluded user account.
In our example, the account USER01 will not apply the configurations from the GPO named MY-GPO.